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Jul 06, 2025
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Website Catalog (In Development)
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CLT 252 - Advanced Histological Techniques
A course focused on the theoretical basis for preparing tissue for histological evaluation and study, including the chemical basis and physical principles of fixation, processing, and staining animal tissue specimens for light microscopy. Emphasis is on routine paraffin techniques, but also includes frozen section techniques, immunohistochemistry, and in situ hybridization.
Prerequisite- Corequisite
Prerequisite: CLT 200/200L Introduction to Histological Techniques
Prerequisite/Corequisite CLT 250/250L Human Histology
Corequisite: CLT 252L Advanced Histological Techniques Lab
Credits: 3
Hours
3 Lecture Hours
Course Profile
Learning Outcomes of the Course:
Upon successful completion of this course the student will be able to:
- State fundamental safety guidelines and practices in the histology laboratory.
- Summarize the theory and practice of techniques used in standard histological preparation and staining of tissues to be evaluated by light microscopy.
- Describe histological procedures for tissue fixation, processing, embedding, sectioning, and staining.
- Describe routine and special stains used to prepare tissue for evaluation.
- Describe rapid diagnostic techniques, immunohistochemical and enzyme histochemical techniques, decalcification, and cytology procedures.
- Recognize possible technical errors and standard troubleshooting techniques.
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