Sep 28, 2023  
Website Catalog (In Development) 
Website Catalog (In Development)

CLT 252 - Advanced Histological Techniques

A lab-oriented course designed to teach students the theory involved in preparing tissue for histological evaluation and study.  Discussions are designed to teach the chemical basis and physical principles of fixation, processing, and staining animal tissue specimens for light microscopy.  The focus will be on routine paraffin techniques, but will include frozen section techniques, immunohistochemistry and in situ hybridization.

Prerequisite- Corequisite
Prerequisite:  CLT 200 Introduction to Histological Techniques

Credits: 3
3 Class Hours
Course Profile
Learning Outcomes of the Course:

Upon successful completion of this course the student will be able to:

  1. Describe proper histological preservation and fixation methods for various tissue specimens, including their classification, impact on specimen, characteristics, and safety concerns.
  2. Describe proper processing of rapid diagnosis or special component preservation as an alternative to paraffin techniques.
  3. Describe the physical and chemical basis for staining histological specimens.
  4. Describe the common stains for each tissue type, including the purpose, preferred fixative, preferred specimen preparation, principle, and control technique.
  5. Describe procedures in specialty areas such as immunohistochemical staining, in situ hybridization techniques, electron microscopy and cytotechnology.