2012-2013 Official General Catalog [Archived Catalog]
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CLT 252 - Advanced Histological Techniques
A lab-oriented course designed to teach students the theory and techniques involved in preparing tissue for histological evaluation and study. Discussions and lab exercises are designed to teach the chemical basis and physical principles of fixation, processing, and staining animal tissue specimans for light microscopy. The focus will be on routine paraffin techniques, but will include frozen section techniques, immunohistochemistry and in situ hybridization.
Prerequisite- Corequisite
Prerequisite: CLT 200 Introduction to Histological Techniques
Credits: 4
Hours
45 Class Hours, 30 Laboratory Hours
Course Profile
Learning Outcomes of the Course:
Upon successful completion of this course the student will be able to:
1. Perform all initial tissue preparation and processing.
- Demonstrate the ability to read and follow directions.
- Demonstrate accuracy and competency in receiving, recording and identifying tissue specimens brought into the lab.
- Demonstrate knowledge of operational principles and components of equipment common to the histology lab.
- Utilize the principles of fixation, decalcification, dehydration, clearing, and infiltration for the successful processing of tissue specimens.
- Explain proper decalcification methods for different applications.
- Demonstrate proper use and care of histological equipment, including microtomes, processors, and staining equipment.
2. Prepare, process, stain, and section tissue samples.
- Demonstrate proper orientation while embedding paraffin processed tissue specimens for routine paraffin sectioning.
- Section paraffin embedded tissues and fresh frozen tissue specimens with precision and accuracy.
- Accurately prepare solutions and stains using good laboratory practice applying concepts of chemistry and mathematics.
- Demonstrate knowledge of the theory and chemistry of staining techniques by successfully preparing stained tissue sections for light microscopic evaluation using routine and special staining procedures.
- Clearly explain the difference between progressive and regressive staining.
- Differentiate nuclear and cytoplasmic components of tissues during routine hematoxylin and eosin staining.
- Successfully monitor and evaluate quality control data.
3. Describe the theory and practice behind various specialized histological practices.
- Explain the difference between histology and cytology.
- Explain what cytotechnology is and demonstrate knowledge of how to prepare slides for cytological examination.
- Perform immunohistochemistry laboratory protocols.
- Define and explain primary and secondary antibodies, immunohistochemistry detection systems and chromagens.
- Compare immunohistochemical techniques such as direct and indirect staining.
- Explain concepts and describe techniques for in situ hybridization.
4. Practice proper laboratory safety procedures.
- Demonstrate knowledge of chemical and biohazard safety practices.
- List the procedures, benefits and advantages of chemical recycling.
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