CLT 258 - Histotechnology Practicum
Performance of procedures in histological technology in an affiliated medical laboratory under direct supervision of medical laboratory personnel. Students will conduct routine histological procedures, develop their laboratory skills, and apply knowledge gained in the program. Emphasis is on cell and tissue morphology, tissue fixation, tissue processing, use and care of a microtome, routine and special histochemical procedures, as well as an introduction to cryotomy, immunohistochemistry, in situ hybridization, and electron microscopy.
Prerequisites: CLT 250 Human Histology, CLT 252 Advanced Histological Techniques
320 Clinical Hours
Learning Outcomes of the Course:
Upon successful completion of this course the student will be able to:
1. Practice accepted procedures for the histology laboratory.
- Demonstrate knowledge of types of histological specimens usually submitted for gross and microscopic examination.
- Document and maintain lab records.
- Promote and contribute to universal bio-safety regulations as well as lab safety.
- Practice quality control in the histology laboratory as required.
2. Perform common tissue processing methods.
- Demonstrate knowledge of human anatomy and physiology as related to histotechnology.
- Demonstrate knowledge of normal histology of human organs.
- Demonstrate knowledge of diagnostic terminology.
- Evaluate, accept, identify and label histologic specimens.
- Demonstrate knowledge of tissue processinig methods, including a variety of fixatives used and how each relates to a specific entity or disease process to be diagnosed.
- Practice proper tissue processing protocols and embedding methods for paraffin block embedding with an understanding of proper dehydration, clearing and infiltrations reagents and methods.
3. Operate and maintain common histological equipment.
- Operate and maintain a tissue processor.
- Operate and maintain a microtome.
- Process, embed, cut, stain, and coverslip all assigned specimens.
- Section, stain and coverslip frozen tissue specimens with a knowledge of cryostat function and maintenance.
- Operate and troubleshoot common laboratory equipment such as chemical recyclers, autostainers, and coverslippers.
- Prepare and stain slides for cytological examination.
4. Evaluate processed, sectioned, and stained tissue.
- Recognize properly differentiated hematoxylin and eosin stained tissue sections.
- Effectively troubleshoot hematoxylin and eosin stains.
- Effectively troubleshoot special stains.
- Evaluate histologic specimens for quality of preparation and staining.
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